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TIME DISKS FLOATING TIME DISKS FLOATING 1 8 2 9 3 10 4 11 5 12 6 13 7 14 Questions for the floating leaf disk lab. Name _____ Legend. The bicarbonate ion serves as the carbon source for photosynthesis. Through the experiment we saw that leaf discs that are not in the presence of sodium bicarbonate no leaf discs floated to the top. However, respiration continues in the dark, so the disks will use the accumulated O 2 gas. Students punch out small discs from leaves, and float them in a syringe of sodium hydrogen carbonate solution. You may have to tap the syringe every 20 to 30 seconds. How many leaves floated per minute? The infiltrated leaves sink in the. This is a … Continue … Bio 210L Lab #6 – Rate of Photosynthesis Page 3 of 5 4. The independent variable for lab 7 is (leaf experiment) The amount of light. Floating Leaf Disk Photosynthesis Lab Introduction: Photosynthesis is a process that converts carbon dioxide into sugars such as glucose using energy from the sun. The data in this lab showed that the spinach leaf disks floated to the top faster in the presence of CO2.When CO 2 was present, the disks started rising at 5 minutes and by the end of the 17 minute trial more than half of them, 11 disks, had risen to the top. Conclusion Because our lab didn't work properly, we weren't able to get proper data to analyze, but using hypothetical data given from a working lab experiment, we would have seen that the water with CO2 added to it was able to allow the leaf disks … T96 Investigation 5. bicarbonate solution. The punched out leaf disks will initially sink in a test tube of water but will float as photosynthesis occurs. The Floating Leaf Disk Lab Introduction: Photosynthesis is a process that converts carbon dioxide into sugars such as glucose using energy from the sun. Photosythesis. If the cup with bicarbonate solution allows the leaf disk to produce oxygen by the process of photosynthesis then the leaf disk in the bicarbonate solution will be the first to float within the 15 minutes allowed. In order to make the leaf disks float, the gases inside were removed by infiltrating them with the bicarbonate and soap solution. HOME Purpose : The purpose of this experiment was to determine the effect of either the presence or absence of carbon dioxide on the rate of photosynthesis in spinach leaves SukYoung Cho IB Biology SL Period 2 Floating Leaf Disk Photosynthesis Lab Introduction Photosynthesis is the process in which plants and bacteria use the energy from sunlight to produce sugar; where cellular respiration converts into ATP. The y-axis shows the number of floating leaf disks. According to our data, the solution of water + sodium bicarbonate has caused the spinach leaves to begin respiration of photosynthesize causing them to rise in the presence of carbon dioxide. o Push on the plunger until only a small volume of air and leaf disk remain in the barrel (< 67 terms. bonkan. Research Question: Do darker colored leaves have a higher photosynthesis rate than lighter colored leaves? The leaf disks placed in the sodium bicarbonate solution did start rising after the first five minutes. When exposed to light, the disks use carbon dioxide and water to produce oxygen and glucose. Present your results in a graph. Earlier you completed the investigative lab, Photo Finish. When the air spaces in the leaf are filled with a sodium bicarbonate solution, the overall density of the leaf disk increases and the disk sinks. WhatsApp logo. Replace the plunger and push out most of the air, but do not crush your leaves. This gives the organism the ability to float. Small disk sections of leaf tissue are punched from a leaf. Get 24⁄7 customer support help when you place a homework help service order with us. Since there was no longer any oxygen in the disk, the spinach leaf has fully photosynthesized in the soap … In photosynthesis, the chemical energy produced through is stored in carbohydrate… During photosynthesis, oxygen is released inside of the leaf which changes the buoyancy--causing the ... Would you expect to see as many floating disks as the intense light dish if you placed the dish 35 or more cm away from the light source? Using a paper single hole puncher, the spinach was punched and many leaf disks were made. Essentially, the assay follows the following steps: 1. syringe, swirl around the leaf disks so they are in the solution. Floating Leaf Disk Photosynthesis Lab. When light is absorbed by pigments in a leaf, the energy absorbed is used to incorporate the carbon dioxide into organic molecules in a process called carbon fixation. Using the leaf disk method, we would test the effect of temperature on the spinach leaves. Photosynthesis: Floating disk lab. The standard procedures such as counting oxygen bubbles generated by an elodea stem tend to not be “student” proof or reliable. The leaf disks got the carbon dioxide needed for photosynthesis from the water that they were submerged in. In the floating leaf disk procedure, a vacuum is used to remove trapped air and infiltrate the interior of plant (leaf) disk samples with a solution containing bicarbonate ions that serve as a carbon source for photosynthesis. Discussion/conclusion. When the air spaces are infiltrated with a solution the overall density of the leaf disk ... of the net rate of photosynthesis. 16 terms. This method is more accurate than to immerse a plant and then count the number of bubbles produced per minute. floating leaf disk photosynthesis lab materials introduction procedures light is part of a continuum of radiation, or energy waves. The water contained carbon dioxide in it, and the leaves performed photosynthesis because light was present and pulled in the carbon dioxide from the water. Control: Spinach disks. Methods, Constants, Controls, & Trials. The Floating Leaf Disk Lab. Photosynthesis how does temperature affect the rate of photosynthesis floating leaf disk lab dataclassroom effect of co2 concentration on photosynthesis lab report carbon dioxide the rate experiment. No matter what kind of academic paper you need, it is simple and affordable to place your order with Achiever Essays. You may need to refer to your o Replace the plunger being careful not to crush the leaf disks. Floating Leaf Disk Assay for Investigating Photosynthesis Pre-Lab questions: answer these in your lab notebook before starting the experiment Name at least four parameters that will affect the rate of photosynthesis. By measuring how many leaf discs are floating at consistent intervals, we can estimate the rate of photosynthesis. As The performance of photosynthesis results in the leaf disk floating to the top of the solution. Repeat this step 2-3 times to get the majority of the disks to sink. Bio 210L Lab #6 – Rate of Photosynthesis Page 3 of 5 4. 45 … Enough spinach leafs were selected to make 12 leaf disks per cup. Include a brief statement describing why you selected the graphing method you did. At minute 10 half of the leaves were floating. biology test (chapter 6) 42 terms. Experiment 3: Measuring the Rate of Photosynthesis with Floating Leaf Discs. This is the point of reference for this procedure. 11 terms. Once students established an understanding of photosynthesis as a chemical reaction, I presented them with the opportunity to create a physical model of the reaction using the leaf disk flotation method ( Appendix A). Light Reactions chain, it gets trapped in the inner spaces of the leaf So as photosynthesis takes place in the leaf disk, tiny oxygen bubbles will push out the solution from the leaf spaces and make the leaf disks light enough to rise. Post-Lab Questions. Label the graph, both axes, and provide a legend to distinguish each trial. Rate of photosynthesis (leaves/min) Spinach: (2-0)/25 = 0.08 B.) Then at minute 20 all of the discs were floating. After the leaf disks have sunk, empty the syringe into a cup and add 3 cm of water. Normally, disks punched out of leaves (with a hole punch) would float. A red line indicates at what time 50% (5) leaf disks float (at about 11.5 minutes). The saturating solution should include a small amount of Sodium bicarbonate. Red and blue light came in second because Bio CH8 Study Guide. In order to measure the rate at which ... photosynthesis Therefore, if more leaf disks rose during the allotted time, the rate of photosynthesis was quicker. The equation for photosynthesis is: To determine As photosynthesis proceeds, oxygen accumulates in the air spaces of the spongy mesophyll and the leaf becomes buoyant and floats. Place them in the syringe and suck in the water. The bubbles should cause the disks to float. Today you will be using an assay, which will allow you to measure the relative rates of photosynthesis. Leaf Disc Assay. Students will write a lab report including, hypothesis, experimental design, data collection / analysis, and conclusion (findings). In Figure 1, spinach leaf disks floated the fastest under white light. The infiltration solution includes a small amount of Sodium bicarbonate. During photosynthesis, plants convert light, water, and carbon dioxide from the air into oxygen and sugars. It is called a Floating Leaf Disk Assay.In this activity you will investigate some of the factors (light intensity) that influence photosynthesis. What caused the leafs to sink during the syringe step? Leaf disks float, normally. They will design and conduct their own experiment to explore the effect of certain factors, such as different environmental variables, on the rate of photosynthesis. The leaf disks under the red light floated slowly at first but sped up as time went on. Whats people lookup in this blog: Leaf Disk Photosynthesis Lab Report; Floating Leaf Disk Photosynthesis Lab Results shorter wavelenghts of energy have greater amounts of energy. After you remove the light and place the cups in the dark, the treatment disks should stop undergoing photosynthesis and the disks should begin to sink. Gross oxygen production and electron transport rates in PSII were uncoupled during the pH perturbation experiments. What was the rate of photosynthesis for each variable? Record the number of disks floating at the end of each minute, swirl the disks to dislodge any that may get stuck to the beaker or to each other. The Floating Leaf Disk Assay for Investigating Photosynthesis ... photosynthesis can occur in a sunken leaf disk. This class helped me expand my lab techniques and exposed me to how researchers use lab equipment to confirm findings. The final assessment for this lab will be a formal lab report recorded in your notebooks to include: hypothesis, experimental design, data collection / analysis, and conclusion (findings). b. the leaf disks. Floating Leaf Disk Photosynthesis Lab. ... whereas green only had 5 leaves float. The control, spinach, had the least amount of leaves floating and therefore the lowest rate of photosynthesis. Conclusion Questions: 1. The process of photosynthesis can be … 6CO2 + 6H20 + Energy (Light) --> C6H12O6 + 6O2 2. The more light, the faster rate of photosynthesis. 1-10 with “ light green ” leaf disks per cup: //www.coursehero.com/file/25707378/Photosynthesis-Lab-Conclusiondocx/ >! 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